![]() Thus, simply remove the background absorbance value from all of the samples, including the controls. Note, the absorbance values I have entered into GraphPad have already been background-corrected. Under the standards, enter the test sample absorbance values, leaving their concentration values blank. The corresponding absorbance values then go in the first ‘ Y‘ column. ![]() ![]() In the ‘ X‘ column, enter the known concentrations of the standards. ![]() Example dataĪs mentioned, for this example I will use data generated from an ELISA experiment. Then click the ‘ Create‘ button to open the new sheet. Then, under the ‘ Enter/import data’ header, select the option ‘ Enter and plot a single Y value for each point‘. When prompted in GraphPad, select an ‘ XY‘ type of Table and Graph sheet. ![]() The aim of this task is to determine the concentration of the test samples by using their abosrbance values and interpolating this from the standard curve. For this example, I have performed an ELISA experiment where I have known concentrations of standards and their absorbance values, as well as some test samples. In this tutorial, I will show you how to interpolate a standard curve by using GraphPad Prism. ![]()
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